The subject of occur ions on amylase activity Aim What is the found of Lead ions on the enzyme Amylase. And does it have an inhibitory effect, which causes the substrate, in this case starch to be blocked from the reply serve up in the enzyme catalyst. Also is the effect reversible or irreversible, which is draw up on the amylase. Method Apparatus and substances required Test tube common carrier 2% starch pa ripage word 6 boil tubes labelled 1 to 6 1% rent treat upshot 6 mental examination tubes labelled A to E, ( F) with child(predicate) chad tiles 5 examen tubes labelled A1, B2, C3, D4, E5, (F6) 5 ml syringe 1% Amylase ascendent 4 dropping pipettes Stop clock Firstly, in for each one of the boiling tubes perplex 8 ml of 2% starch solution exploitation the syringe. therefore in each of the demonstrate tubes labelled A to F add 1ml of 1% Amylase solution. In the remaining 6 rise tubes add the different concentrations of the stimulate in treat solution as shown below in their corresponding test tubes. All concentrations ar achieved by serial dilution in the beginning coming from a 1% adept treat solution. Overall Concentration of Lead nitrate in solution A1 - 1ml manoeuvre nitrate solution (1%) 1% B2 - 0.8 ml of 1% protract nitrate solution, 0.2 ml of distilled body of irrigate 0.8% C3 - 0.
6 ml of 1% engineer nitrate solution, 0.4 ml of distilled water 0.6% D4 - 0.4ml of 1% lead nitrate solution, 0.6 ml of distilled water 0.4% E5 - 0.2 ml of 1% lead nitrate solution, 0.8 ml of distilled water 0.2% F6 - N/A N/A After these are all measured place accurately fill pregnant chad tiles with iodine solution. To start the reaction mix the two test tubes, A and A1 in to boiling tube, 1mix well and start the clock, and every... If you exact to get a estimable essay, order it on our website:
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